VALIDATION IMAGES
Lane 1: Hela; Lane 2: MCF-7; Lane 3: Human liver lysate robed with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:1000 overnight at 4C followed by a conjugated secondary antibody for 60 minutes at 37C.
Flow cytometric analysis of HeLa cells with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse prostate tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Mouse Testis lysates; Lane 2: Rat Testis lysates; Lane 3: Mouse Spleen lysates; Lane 4: Rat Spleen lysates; Lane 5: Mouse Lung lysates; Lane 6: Rat Lung lysates; Lane 7: Human MCF-7 cell lysates; Lane 8: Human Hela cell lysates; Lane 9: Human Raji cell lysates probed with Bax (3C3) Monoclonal Antibody, Unconjugated (bsm-52316R ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.