bsm-52316R [Primary Antibody]
Bax (3C3) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Bax

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 581

Swiss Prot: Q07812

Source: Recombinant human Bak between 20 to 150 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 21,15 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shuang Huet al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci. 2020 Oct 1;258:118147.Read more>>
  • Chenying Gao. et al. Inhibition of the Ras/ERK1/2 pathway contributes to the protective effect of ginsenoside Re against intimal hyperplasia. 2021 May 2Read more>>
  • Wang, Sanchun. et al. Pseudoginsengenin DQ exerts antitumour activity against hypopharyngeal cancer cells by targeting the HIF-1-GLUT1 pathway. Cancer Cell Int. 2021 Dec;21(1):1-12Read more>>
  • Xiaojing Zhang. et al. A new discovery: Total Bupleurum saponin extracts can inhibit the proliferation and induce apoptosis of colon cancer cells by regulating the PI3K/Akt/mTOR pathway. J Ethnopharmacol. 2022 Jan;283:114742Read more>>
  • Yanji Zhu. et al. P2X7R antagonist protects against renal injury in mice with adriamycin nephropathy. Exp Ther Med. 2022 Feb;23(2):1-8Read more>>
  • Chao Wang. et al. Inhibition of monoacylglycerol lipase restrains proliferation, migration, invasion, tumor growth and induces apoptosis in cervical cancer. 2021 Dec 07Read more>>
  • Zhuohong Li. et al. Anti-inflammatory and anti-apoptotic effects of Shaoyao decoction on X-ray radiation-induced enteritis of C57BL/6 mice. J Ethnopharmacol. 2022 Mar;:115158Read more>>
  • Yu-Sheng Shi. et al. Pteris laeta Wall. and Its New Phytochemical, Pterosinsade A, Promote Hippocampal Neurogenesis via Activating the Wnt Signaling Pathway. J AGR FOOD CHEM. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Miao Song. et al. Mitophagy alleviates AIF-mediated spleen apoptosis induced by AlCl3 through Parkin stabilization in mice. FOOD CHEM TOXICOL. 2023 Jun;176:113762Read more>>
  • Fangji Zhou. et al. Mono-(2-ethylhexyl) Phthalate (MEHP)-Induced Telomere Structure and Function Disorder Mediates Cell Cycle Dysregulation and Apoptosis via c-Myc and Its Upstream Transcription Factors in a Mouse Spermatogonia-Derived (GC-1) Cell Line. TOXICS. 2023 May;11(5):448Read more>>
  • Gan Rao. et al. Arsenic and polystyrene-nano plastics co-exposure induced testicular toxicity: Triggers oxidative stress and promotes apoptosis and inflammation in mice. ENVIRON TOXICOL. 2023 SepRead more>>
VALIDATION IMAGES

Lane 1: Hela; Lane 2: MCF-7; Lane 3: Human liver lysate robed with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:1000 overnight at 4C followed by a conjugated secondary antibody for 60 minutes at 37C.


Flow cytometric analysis of HeLa cells with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse prostate tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Bax (3C3) Monoclonal Antibody (bsm-52316R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Mouse Testis lysates; Lane 2: Rat Testis lysates; Lane 3: Mouse Spleen lysates; Lane 4: Rat Spleen lysates; Lane 5: Mouse Lung lysates; Lane 6: Rat Lung lysates; Lane 7: Human MCF-7 cell lysates; Lane 8: Human Hela cell lysates; Lane 9: Human Raji cell lysates probed with Bax (3C3) Monoclonal Antibody, Unconjugated (bsm-52316R ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.