VALIDATION IMAGES
Lane 1: Hela; Lane 2: Jurkat; Lane 3: K562 lysate probed with ERK1 (6B4) Monoclonal Antibody (bsm-52329R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of Jurkat cells with ERK1 (6B4) Monoclonal Antibody (bsm-52329R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with ERK1 (6B4) Monoclonal Antibody (bsm-52329R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with ERK1 (6B4) Monoclonal Antibody (bsm-52329R) at 1:300 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with ERK1 (6B4) Monoclonal Antibody (bsm-52329R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.