bsm-52336R [Primary Antibody]
PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody
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Host: Rabbit

Target Protein: PHD1/prolyl hydroxylase

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 112398

Swiss Prot: Q96KS0

Source: Recombinant human PHD1/prolyl hydroxylase between 1 to 100 amino acids.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferentially recognized via a LXXLAP motif.

Size: 100uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
IHC(1:50-200)

Predicted Molecular Weight: 44 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela; Lane 2: PC12; Lane 3: NIH/3T3 lysate probed with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of Hela cells with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


IF(ICC) staining with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Mouse prostate tissue incubated with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with PHD1/prolyl hydroxylase (7G8) Monoclonal Antibody (bsm-52336R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.