bsm-52337R [Primary Antibody]
PMS2 (9H11) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PMS2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5395

Swiss Prot: P54278

Source: Full length protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 96


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Hela cell lysates probed with PMS2 (9H11) Monoclonal Antibody (bsm-52337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.


Lane 1: Jurkat cell lysates;Lane 2: Hela cell lysates; Lane 3: SK-BR-3 cell lysates probed with PMS2 Polyclonal Antibody, Unconjugated (bsm-52337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Flow cytometric analysis of HeLa cells with PMS2 (9H11) Monoclonal Antibody (bsm-52337R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).


IF(ICC) staining with PMS2 (9H11) Monoclonal Antibody (bsm-52337R) at 1:100 in HeLa cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PMS2 (9H11) Monoclonal Antibody (bsm-52337R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.