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Host: Rabbit
Target Protein: Melan A
Clonality: Recombinant
Isotype: IgG
Entrez Gene: 2315
Swiss Prot: Q16655
Source: A synthesized peptide derived from human MART1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: Lot dependent
Predicted Molecular Weight: 13
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Human melanoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Melan A Monoclonal Antibody, Unconjugated(bsm-52337R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with MART1 monoclonal antibody, unconjugated (bsm-52337R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.