VALIDATION IMAGES
Hela cell lysates probed with PMS2 (9H11) Monoclonal Antibody (bsm-52337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Lane 1: Jurkat cell lysates;Lane 2: Hela cell lysates; Lane 3: SK-BR-3 cell lysates probed with PMS2 Polyclonal Antibody, Unconjugated (bsm-52337R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Flow cytometric analysis of HeLa cells with PMS2 (9H11) Monoclonal Antibody (bsm-52337R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with PMS2 (9H11) Monoclonal Antibody (bsm-52337R) at 1:100 in HeLa cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PMS2 (9H11) Monoclonal Antibody (bsm-52337R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.