bsm-52344R [Primary Antibody]
FUBP1 (3F12) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: FUBP1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 8880

Swiss Prot: Q96AE4

Source: Recombinant protein within Human FUBP1 between 600 amino acids to C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat
(WB: 1:1,000-1:2,000; ICC: 1:100-1:500; IHC: 1:50-1:200; FC: 1:50-1:100)

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cell lysates; Lane 2: Raji cell lysates probed with FUBP1 (3F12) Monoclonal Antibody, Unconjugated (bsm-52344R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Flow cytometric analysis of Jurkat cells with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


IF(ICC) staining with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse pancreas tissue incubated with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with FUBP1 (3F12) Monoclonal Antibody (bsm-52344R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.