VALIDATION IMAGES
Lane 1: Mouse Liver lysates; Lane 2: Mouse Brain lysates probed with Lamin B1 (4A3) Monoclonal Antibody (bsm-52361R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Lamin B1 (4A3) Monoclonal Antibody (bsm-52361R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with Lamin B1 (4A3) Monoclonal Antibody (bsm-52361R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Lamin B1 (4A3) Monoclonal Antibody (bsm-52361R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Lamin B1 (4A3) Monoclonal Antibody (bsm-52361R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Lamin B1 (4A3) Monoclonal Antibody (bsm-52361R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Rat Cerebrum tissue lysates ; Lane 5: Rat Pancreas tissue lysates; Lane 6: Rat Testis tissue lysates; Lane 7: Human U937 cell lysates; Lane 8: Human Jurkat cell lysates; Lane 9: Human Molt-4 cell lysates; Lane 10: Human Hela cell lysates probed with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52316R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Lamin B1 (4A3) Monoclonal Antibody, Unconjugated (bsm-52361R) at 1:200 overnight at 4°C, DAB staining.