bsm-52362R [Primary Antibody]
NGF Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NGF

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4803

Swiss Prot: P01138

Source: Recombinded human NGF full length protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 32 kDa


Cross Reactive Species: Human
Mouse
Rat
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Hela cell lysates probed with NGF (11G1) Monoclonal Antibody (bsm-52362R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with NGF (11G1) Monoclonal Antibody (bsm-52362R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with NGF (11G1) Monoclonal Antibody (bsm-52362R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Heart lysates; Lane 4: Rat Heart lysates; Lane 5: Rat Spleen lysates; Lane 6: Mouse Placenta lysates probed with NGF (11G1) Monoclonal Antibody, Unconjugated (bsm-52362R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.