bsm-52368R [Primary Antibody]
BDNF (2A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: BDNF

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 627

Swiss Prot: P23560

Source: Recombinant human BDNF between 1 to 100 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 28 kDa


Cross Reactive Species: Human
Mouse
Rat
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Pallavi Gurung. et al. Euonymus alatus Twig Extract Protects against Scopolamine-Induced Changes in Brain and Brain-Derived Cells via Cholinergic and BDNF Pathways. NUTRIENTS. 2023 Jan;15(1):128Read more>>
  • Xiaoyu Ma. et al. Multifunctional injectable hydrogel promotes functional recovery after stroke by modulating microglial polarization, angiogenesis and neuroplasticity. CHEM ENG J. 2023 May;464:14252Read more>>
VALIDATION IMAGES

Lane 1: SHG-44 cell lysates; Lane 2: A172 cell lysates; Lane 3: Mouse brain lysates probed with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.


Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Cerebellum lysates; Lane 4: Rat Cerebellum lysates; Lane 5: Mouse Muscle lysates; Lane 6: Rat Muscle lysates; Lane 7: Mouse Heart lysates; Lane 8: Rat Heart lysates; Lane 9: Mouse Testis lysates; Lane 10: Rat Testis lysates; Lane 11: Human Hela cell lysates; Lane 12: Human U251 cell lysates; Lane 13: Human A549 cell lysates; Lane 14: Human U-2OS cell lysates probed with BDNF (2A1) Monoclonal Antibody, Unconjugated (bsm-52368R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.