VALIDATION IMAGES
Lane 1: SHG-44 cell lysates; Lane 2: A172 cell lysates; Lane 3: Mouse brain lysates probed with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Cerebellum lysates; Lane 4: Rat Cerebellum lysates; Lane 5: Mouse Muscle lysates; Lane 6: Rat Muscle lysates; Lane 7: Mouse Heart lysates; Lane 8: Rat Heart lysates; Lane 9: Mouse Testis lysates; Lane 10: Rat Testis lysates; Lane 11: Human Hela cell lysates; Lane 12: Human U251 cell lysates; Lane 13: Human A549 cell lysates; Lane 14: Human U-2OS cell lysates probed with BDNF (2A1) Monoclonal Antibody, Unconjugated (bsm-52368R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.