VALIDATION IMAGES
Lane 1: MCF-7 cell lysates;Lane 2: Hela cell lysates; Lane 3: 293T cell lysates probed with PTEN (9D2) Monoclonal Antibody, Unconjugated (bsm-52369R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
IF(ICC) staining with PTEN (9D2) Monoclonal Antibody (bsm-52369R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PTEN (9D2) Monoclonal Antibody (bsm-52369R) at 1:300 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with PTEN (9D2) Monoclonal Antibody (bsm-52369R) at 1:300 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PTEN (9D2) Monoclonal Antibody (bsm-52369R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with PTEN (9D2) Monoclonal Antibody (bsm-52369R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human SH-SY5Y cell lysates probed with PTEN Monoclonal Antibody, Unconjugated (bsm-52369R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.