bsm-52373R [Primary Antibody]
MMP14 (3A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MMP14

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4323

Swiss Prot: P50281

Source: Recombinant human MMP14 between 150 to 250 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 65 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Human kidney lysates probed with MMP14 (3A1) Monoclonal Antibody (bsm-52373R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.


Flow cytometric analysis of A549 lysate probed with MMP14 (3A1) Monoclonal Antibody (bsm-52373R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with MMP14 (3A1) Monoclonal Antibody (bsm-52373R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with MMP14 (3A1) Monoclonal Antibody (bsm-52373R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with MMP14 (3A1) Monoclonal Antibody (bsm-52373R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.