bsm-52374R [Primary Antibody]
Integrin beta 3 (2B11) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ITGB3

Immunogen Range: 729-788/788


Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3690

Swiss Prot: P05106

Source: Recombinant Human Integrin beta 3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: HUVEC cell lysates; Probed with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at a 1:1000 dilution, overnight at 4˚C.


IF(ICC) staining with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue).


IF(ICC) staining with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue).


Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Integrin beta 3 (2B11) Monoclonal Antibody (bsm-52374R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.