bsm-52375R [Primary Antibody]
CD86 Recombinant Antibody
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Host: Rabbit

Target Protein: CD86

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 942

Swiss Prot: P42081

Source: Recombinant human CD86 between 1 to 200 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Store at -20°C for 12 months.

Background:

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

Size: 100uL

Concentration: Lot Dependent

Applications: WB(1:100-200)
Flow-Cyt(1:500-2000)
ICC/IF(1:100-200)

Predicted Molecular Weight: 70 kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Peng Xue. et al. Fe3+ mediated shikonin and PPA coloaded liposomes induce robust immunogenic cell death by integrating ROS enhancement and GSH depletion. INT J PHARMACEUT. 2024 Jan;649:123657Read more>>
  • Xiaoyue Guan. et al. The Role of Macrophage Efferocytosis in the Pathogenesis of Apical Periodontitis. INT J MOL SCI. 2024 Jan;25(7):3854Read more>>
VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with CD86 monoclonal antibody, unconjugated (bsm-52375R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (CD86) monoclonal Antibody, unconjugated (bsm-52375R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.