bsm-52381R [Primary Antibody]
Apaf-1 (4G2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Apaf-1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 317

Swiss Prot: O14727

Source: Recombinant human Apaf-1 between 1 to 300 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 141 kDa


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

HUVEC cell lysates probed with Apaf-1 (4G2) Monoclonal Antibody (bsm-52381R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.


IF(ICC) staining with Apaf-1 (4G2) Monoclonal Antibody (bsm-52381R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Apaf-1 (4G2) Monoclonal Antibody (bsm-52381R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Apaf-1 (4G2) Monoclonal Antibody (bsm-52381R) at 1:100 in AGS cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with Apaf-1 (4G2) Monoclonal Antibody (bsm-52381R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Apaf-1 (4G2) Monoclonal Antibody (bsm-52381R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with Apaf-1 (4G2) Monoclonal Antibody (bsm-52381R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Apaf-1 (4G2) Monoclonal Antibody (bsm-52381R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.