bsm-52402R [Primary Antibody]
HAPLN1 (30A1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HAPLN1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 1404

Swiss Prot: P10915

Source: Recombinant protein of Human HAPLN1 between 300 amino acids to the C-terminus.

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 40kDa


Cross Reactive Species: Human
Mouse
Rat
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: BT-20 cell lysates; Lane 2: HUVEC cell lysates; Lane 3: SW480 cell lysates probed with HAPLN1 (30A1) Monoclonal Antibody, Unconjugated (bsm-52402R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.


IF(ICC) staining with HAPLN1 (30A1) Monoclonal Antibody (bsm-52402R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with HAPLN1 (30A1) Monoclonal Antibody (bsm-52402R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with HAPLN1 (30A1) Monoclonal Antibody (bsm-52402R) at 1:100 in BT-20 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with HAPLN1 (30A1) Monoclonal Antibody (bsm-52402R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with HAPLN1 (30A1) Monoclonal Antibody (bsm-52402R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with HAPLN1 (30A1) Monoclonal Antibody (bsm-52402R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.