bsm-52408R [Primary Antibody]
Cleaved PARP (2A10) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cleaved PARP

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 142

Swiss Prot: P09874

Source: Synthetic peptide within Human PARP1 aa 150-250

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008].

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Luya Pu. et al. Icariin arrests cell cycle progression and induces cell apoptosis through the mitochondrial pathway in human fibroblast-like synoviocytes. Eur J Pharmacol. 2021 Dec;912:174585Read more>>
  • Kunlin Wu. et al. Histone deacetylase inhibitor panobinostat in combination with rapamycin confers enhanced efficacy against triple-negative breast cancer. EXP CELL RES. 2022 Sep;:113362Read more>>
  • Miao Song. et al. Mitophagy alleviates AIF-mediated spleen apoptosis induced by AlCl3 through Parkin stabilization in mice. FOOD CHEM TOXICOL. 2023 Jun;176:113762Read more>>
VALIDATION IMAGES

Lane 1: Jurkat cell; Lane 2: A549 cell; probed with Cleaved PARP (2A10) Monoclonal Antibody (bsm-52408R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Flow cytometric analysis of HeLa cells with Cleaved PARP (2A10) Monoclonal Antibody (bsm-52408R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: Human Jurkat cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human HL60 cell lysates; Lane 4: Human K562 cell lysates probed with Cleaved PARP Monoclonal Antibody, Unconjugated (bsm-52408R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.