bsm-52410R
[Primary Antibody]
PKC alpha (2A1) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: PKC alpha
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 5578
Swiss Prot: P17252
Source: C-terminus human PKC alpha
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: K562 cell; Lane 2: 293 cell; Probed with PKC alpha (2A1) Monoclonal Antibody (bsm-52410R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of HeLa cells with PKC alpha (2A1) Monoclonal Antibody (bsm-52410R) at a 1:50 dilution (blue) compared with an unlabeled control (cells without incubation with primary antibody; red).
IF(ICC) staining with PKC alpha (2A1) Monoclonal Antibody (bsm-52410R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with PKC alpha (2A1) Monoclonal Antibody (bsm-52410R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with PKC alpha (2A1) Monoclonal Antibody (bsm-52410R) at 1:100 in A549 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse intestine tissue incubated with PKC alpha (2A1) Monoclonal Antibody (bsm-52410R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with PKC alpha (2A1) Monoclonal Antibody (bsm-52410R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with PKC alpha (2A1) Monoclonal Antibody (bsm-52410R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.