bsm-52415R
[Primary Antibody]
Bak (7A12) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Bak
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 578
Swiss Prot: Q16611
Source: Human Bak between 1 to 100 amino acids
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Plays a role in the mitochondrial apoptosic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Hela cells; Lane 2: Human skeletal muscle; Lane 3: Ags cells; Probed with Bak (7A12) Monoclonal Antibody (bsm-52415R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of NIH/3T3 cells with Bak (7A12) Monoclonal Antibody (bsm-52415R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Bak (7A12) Monoclonal Antibody (bsm-52415R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with Bak (7A12) Monoclonal Antibody (bsm-52415R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.