VALIDATION IMAGES
Lane 1: A431 cells; Lane 2: 293 cells; Lane 3: MCF-7 cells; Probed with PARP (45G1) Monoclonal Antibody (bsm-52433R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Flow cytometric analysis of Hela cells with PARP (45G1) Monoclonal Antibody (bsm-52433R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with PARP (45G1) Monoclonal Antibody (bsm-52433R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with PARP (45G1) Monoclonal Antibody (bsm-52433R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with PARP (45G1) Monoclonal Antibody (bsm-52433R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.