bsm-52434R-Cy5.5

DATASHEET

Host: Rabbit

Target Protein: Lactate Dehydrogenase

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3939

Swiss Prot: P00338

Source: Human Lactate Dehydrogenase between 250 to C-terminus

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 8489% sequence similarities and 6975% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers.

Conjugation: Cy5.5

Excitation/ Emission: 675nm/694nm

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:500-2000)
IF(1:100-200)
Flow-Cyt(1:50-100)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.