bsm-52435R [Primary Antibody]
Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody
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Host: Rabbit

Target Protein: Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139)

Modification Site: PAK1 (S144)+PAK2 (S141)+PAK3 (S139)

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5063

Swiss Prot: O75914

Source: Synthetic phospho-peptide surrounding S139+S141+S144 of PAK

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IP(1-2ug)
IF
Flow-Cyt(1ug/Test)
ICC/IF(1:50-100)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cells; Lane 2: NIH/3T3 cells; Lane 3: SH-SY-5Y cells; Probed with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.