bsm-52435R-PE-Cy5.5

DATASHEET

Host: Rabbit

Target Protein: Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139)

Modification Site: PAK1 (S144)+PAK2 (S141)+PAK3 (S139)

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 5063

Swiss Prot: O75914

Source: Synthetic phospho-peptide surrounding S139+S141+S144 of PAK

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.

Conjugation: PE-Cy5.5

Excitation/ Emission: 488nm/694nm

Size: 100uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IP(1-2ug)
IF(1:50-200)
Flow-Cyt(1ug/Test)
ICC/IF(1:50-100)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.