bsm-52436R-BF488 [Conjugated Primary Antibody]
Calreticulin Recombinant Antibody, AbBy Fluor-488 Conjugated
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Host: Rabbit

Target Protein: Calreticulin

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 811

Swiss Prot: P27797

Source: A synthesized peptide derived from human Calreticulin

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Conjugation: AbBy Fluor-488

Excitation/ Emission: 499nm/519nm

Size: 100µL

Concentration: 1mg/ml

Applications: WB(WB(1:2000-10000))
FCM(FCM(1:50-100))
IHC-P(IHC-P(1:200-800))
IHC-F(IHC-F(1:200-800))
IF(IHC-P)(IF(IHC-P)(1:200-800))
IF(ICC)(IF(ICC)(1:100-200))

Predicted Molecular Weight: 44


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Guoyun Wan. et al. Endoplasmic reticulum-targeted NIR-II phototherapy combined with inflammatory vascular suppression elicits a synergistic effect against TNBC. BIOMATER SCI-UK. 2023 JaRead more>>
  • ingchen Zhang. et al. Precise RNA Editing: Cascade Self-Uncloaking Dual-Prodrug Nanoassemblies Based on CRISPR/Cas13a for Pleiotropic Immunotherapy of PD-L1-Resistant Colorectal Cancer. ADV FUNCT MATER. 2023 Sep;:230563Read more>>
VALIDATION IMAGES

The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green): BF488 conjugated Rabbit Anti-Calreticulin antibody (bsm-52436R-BF488,1:100);Isotype Control (orange): Rabbit IgG/BF488 (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.