VALIDATION IMAGES
Flow cytometric analysis of HepG2 cells with Ubiquitin (7C6) Monoclonal Antibody (bsm-52447R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).
Mouse heart lysate probed with Ubiquitin (7C6) Monoclonal Antibody (bsm-52447R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Ubiquitin (7C6) Monoclonal Antibody (bsm-52447R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Ubiquitin (7C6) Monoclonal Antibody (bsm-52447R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Ubiquitin (7C6) Monoclonal Antibody (bsm-52447R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse placenta tissue incubated with Ubiquitin (7C6) Monoclonal Antibody (bsm-52447R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Ubiquitin (7C6) Monoclonal Antibody (bsm-52447R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.