bsm-52448R
[Primary Antibody]
ASH2L (1B6) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: ASH2L
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 9070
Swiss Prot: Q9UBL3
Source: C-terminus human ASH2L
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: PC12 cells; Lane 2: 293T cells; Probed with ASH2L (1B6) Monoclonal Antibody (bsm-52448R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with ASH2L (1B6) Monoclonal Antibody (bsm-52448R) at 1:300 in PC-12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with ASH2L (1B6) Monoclonal Antibody (bsm-52448R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse ovary tissue incubated with ASH2L (1B6) Monoclonal Antibody (bsm-52448R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with ASH2L (1B6) Monoclonal Antibody (bsm-52448R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse placenta tissue incubated with ASH2L (1B6) Monoclonal Antibody (bsm-52448R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with ASH2L (1B6) Monoclonal Antibody (bsm-52448R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.