bsm-52449R
[Primary Antibody]
TOMM20 (32A1) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: TOMM20
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 9804
Swiss Prot: Q15388
Source: C-terminus Human TOMM20
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).
Lane 1: Hela cells; Lane 2: MCF-7 cells; Lane 3: F9 cells; Lane 4: PC12 cells; Probed with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100 in PC-12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with TOMM20 (32A1) Monoclonal Antibody (bsm-52449R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.