VALIDATION IMAGES
Lane 1: Hela cells; Lane 2: 293 cells; Lane 3: MCF-7 cells; Lane 4: HepG2 cells; Probed with Hexokinase 1 (7B9) Monoclonal Antibody (bsm-52452R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Hexokinase 1 (7B9) Monoclonal Antibody (bsm-52452R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Hexokinase 1 (7B9) Monoclonal Antibody (bsm-52452R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Hexokinase 1 (7B9) Monoclonal Antibody (bsm-52452R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with Hexokinase 1 (7B9) Monoclonal Antibody (bsm-52452R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with Hexokinase 1 (7B9) Monoclonal Antibody (bsm-52452R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with Hexokinase 1 (7B9) Monoclonal Antibody (bsm-52452R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Hexokinase 1 (7B9) Monoclonal Antibody (bsm-52452R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.