bsm-52455R-APC [Conjugated Primary Antibody]
Ki-67 Recombinant Antibody, APC Conjugated
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Host: Rabbit

Target Protein: Ki-67

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 4288

Swiss Prot: P46013

Source: KLH conjugated synthetic peptide derived from human Ki67

Purification: purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Conjugation: APC

Excitation/ Emission: 650nm/660nm

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:1000-5000)
IHC-F(1:200-1000)
IHC-P(1:200-1000)
ICC/IF(1:200-1000)
IF(1:200-1000)
Flow-Cyt(1:100-200)

Predicted Molecular Weight: 358 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Ki-67) monoclonal Antibody,APC conjugated (bsm-52455R-APC) 1:100, 90 min at 37°C; DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.