bsm-52462R
[Primary Antibody]
Phospho-JNK1/2/3 (T183+T183+T221) (6C10) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Phospho-JNK1/2/3 (T183+T183+T221)
Modification Site: T183+T183+T221
Clonality: Monoclonal
Isotype: IgG
Source: Synthetic phospho-peptide surrounding Thr183 + Thr183 + Thr221 of human JNK1/2/3
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun N-terminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Mei-Hua Jin. et al. Hispidin inhibits LPS?induced nitric oxide production in BV?2 microglial cells via ROS?dependent MAPK signaling. Exp Ther Med. 2021 Sep;22(3):1-9Read more>>
- Lemiao Zhong. et al. Mycoplasma synoviae induce spleen tissue damage and inflammatory response of chicken through oxidative stress and apoptosis. VIRULENCE. 2023 Nov 16Read more>>
- Xinyun Qin. et al. Regulation of the intestinal flora using polysaccharides from Callicarpa nudiflora Hook to alleviate ulcerative colitis and the molecular mechanisms involved. INT J BIOL MACROMOL. 2024 Feb;258:128887Read more>>
VALIDATION IMAGES
Lane 1: NIH/3T3 cell lysate, treated with Anisomycin; Lane 2: NIH/3T3 cell lysate, untreated; Probed with Phospho-JNK1/2/3 (T183+T183+T221) (6C10) Monoclonal Antibody (bsm-52462R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Phospho-JNK1/2/3 (T183+T183+T221) (6C10) Monoclonal Antibody (bsm-52462R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Phospho-JNK1/2/3 (T183+T183+T221) (6C10) Monoclonal Antibody (bsm-52462R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Phospho-JNK1/2/3 (T183+T183+T221) (6C10) Monoclonal Antibody (bsm-52462R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with Phospho-JNK1/2/3 (T183+T183+T221) (6C10) Monoclonal Antibody (bsm-52462R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Phospho-JNK1/2/3 (T183+T183+T221) (6C10) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Phospho-JNK1/2/3 (T183+T183+T221) (6C10) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Phospho-JNK1/2/3 (T183+T183+T221) (6C10) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human endometrial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Phospho-JNK1/2/3 (T183+T183+T221) (6C10) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:200 overnight at 4°C, DAB staining.