bsm-52462R [Primary Antibody]
Phospho-JNK1/2/3(T183+T183+T221) Recombinant Antibody
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Host: Rabbit

Target Protein: Phospho-JNK1/2/3(T183+T183+T221)

Modification Site: T183+T183+T221

Clonality: Recombinant

Isotype: IgG

Source: Synthetic phospho-peptide surrounding Thr183 + Thr183 + Thr221 of human JNK1/2/3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

Storage: Shipped at 4°. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun N-terminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.

Size: 100µL

Concentration: Lot dependent

Applications: IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))
IF(IHC-P)(IF(IHC-P)(1:100-500))
IF(ICC)(IF(ICC)(1:50-200))

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Mei-Hua Jin. et al. Hispidin inhibits LPS?induced nitric oxide production in BV?2 microglial cells via ROS?dependent MAPK signaling. Exp Ther Med. 2021 Sep;22(3):1-9Read more>>
  • Lemiao Zhong. et al. Mycoplasma synoviae induce spleen tissue damage and inflammatory response of chicken through oxidative stress and apoptosis. VIRULENCE. 2023 Nov 16Read more>>
  • Xinyun Qin. et al. Regulation of the intestinal flora using polysaccharides from Callicarpa nudiflora Hook to alleviate ulcerative colitis and the molecular mechanisms involved. INT J BIOL MACROMOL. 2024 Feb;258:128887Read more>>
  • Keyi Nong. et al. Potential effects and mechanism of flavonoids extract of Callicarpa nudiflora Hook on DSS-induced colitis in mice. PHYTOMEDICINE. 2024 Jun;128:155523Read more>>
  • Zhao Hao-Ran. et al. Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE/ Mice. CELL BIOCHEM BIOPHYS. 2024 Aug;:1-23Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-JNK1/2/3(T183+T183+T221) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-JNK1/2/3(T183+T183+T221) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-JNK1/2/3(T183+T183+T221) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-JNK1/2/3(T183+T183+T221) Monoclonal Antibody, Unconjugated (bsm-52462R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


4% Paraformaldehyde-fixed Hela (treated with 100 ng/ml EGF for 20 mins) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Phospho-JNK1/2/3(T183+T183+T221)) monoclonal Antibody, unconjugated (bsm-52462R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.


Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-JNK1 2 3(T183+T183+T221) Monoclonal Antibody, Unconjugated(bsm-52462R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.