bsm-52462R-Biotin

DATASHEET

Host: Rabbit

Target Protein: Phospho-JNK1/2/3(T183+T183+T221)

Modification Site: T183+T183+T221

Clonality: Recombinant

Isotype: IgG

Source: Synthetic phospho-peptide surrounding Thr183 + Thr183 + Thr221 of human JNK1/2/3

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun N-terminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100µL

Concentration: Lot dependent

Applications: IHC-P(IHC-P(1:100-500))
IHC-F(IHC-F(1:100-500))

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.