bsm-52463R

DATASHEET

Host: Rabbit

Target Protein: PRMT5

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 10419

Swiss Prot: O14744

Source: Human PRMT5 between 50-150 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

DescriptionThe formation of the spliceosome includes the assembly of Sm proteins in an ordered manner onto snRNAs. This process is mediated by the survival of a motor neuron (SMN) protein and is enhanced by modification of specific Arginine residues in the Sm proteins to symmetrical dimethylarginines (sDMAs). sDMA modification of Sm proteins is catalyzed by the methylosome, a complex comprised of the type II methyltransferase PRMT5, also designated JAK-binding protein 1), (JBP1), pICln, and two novel factors. PRMT5 binds the Sm proteins via their Arginine- and Glycine-rich (RG) domains, while pICln binds the Sm domains. PRMT5 is a distinct member of the protein-Arginine methyltransferase (PRMT) family, and predominantly localizes to the cytoplasm in a wide variety of tissues. PRMT5 also associates specifically with the transcription start site region of the cyclin E1 promoter, and, therefore, is involved in the control of transcription and proliferation. The gene encoding human PRMT5 maps to chromosome 14q11.

Size: 100uL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
IHC(1:50-200)

Predicted Molecular Weight: 73 kDa

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.