bsm-52466R [Primary Antibody]
ICAM1 (1G2) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ICAM1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3383

Swiss Prot: P05362

Source: Recombinant human ICAM1 between 1-100 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Hela cells; Lane 2: HUVEC cells; Lane 3: Raji cells; Probed with ICAM1 (1G2) Monoclonal Antibody (bsm-52434R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with ICAM1 (1G2) Monoclonal Antibody (bsm-52466R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with ICAM1 (1G2) Monoclonal Antibody (bsm-52466R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with ICAM1 (1G2) Monoclonal Antibody (bsm-52466R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with ICAM1 (1G2) Monoclonal Antibody (bsm-52466R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with ICAM1 (1G2) Monoclonal Antibody (bsm-52466R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with ICAM1 (1G2) Monoclonal Antibody (bsm-52466R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.