bsm-52466R-Biotin [Conjugated Primary Antibody]
ICAM1 Recombinant Antibody, Biotin Conjugated
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Host: Rabbit

Target Protein: ICAM1

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 3383

Swiss Prot: P05362

Source: A synthesized peptide derived from human ICAM 1

Purification: purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:1000-5000)
IHC-F(1:200-1000)
IF(1:200-1000)
IHC-P(1:200-1000)

Predicted Molecular Weight: 56 kDa


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ICAM1 Monoclonal Antibody (bsm-52466R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ICAM1 Monoclonal Antibody (bsm-52466R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ICAM1 Monoclonal Antibody (bsm-52466R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ICAM1 Monoclonal Antibody (bsm-52466R-Bio) at 1:200 overnight at 4°C, and DAB (C-0010) staining.