bsm-52469R [Primary Antibody]
CD4 Recombinant Antibody
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Host: Rabbit

Target Protein: CD4

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 920

Swiss Prot: P01730

Source: A synthesized peptide derived from human CD4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µL

Concentration: 1mg/ml

Applications: WB(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF
Flow-Cyt(1ug/Test)

Predicted Molecular Weight: 48


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhu Li. et al. Immunogenic Cell Death Augmented by Manganese Zinc Sulfide Nanoparticles for Metastatic Melanoma Immunotherapy. ACS NANO. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Guoyun Wan. et al. Endoplasmic reticulum-targeted NIR-II phototherapy combined with inflammatory vascular suppression elicits a synergistic effect against TNBC. BIOMATER SCI-UK. 2023 JaRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Polyclonal Antibody, Unconjugated (bsm-52469R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-52469R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-52469R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining


Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-52469R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-52469R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining


Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-52469R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated (bsm-52469R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Lung Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-52469R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated (bsm-52469R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Colon ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-52469R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with CD4 monoclonal antibody, unconjugated (bsm-52469R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD4 Monoclonal Antibody, Unconjugated (bsm-52469R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD4 Monoclonal Antibody, Unconjugated(bsm-52469R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD4 Monoclonal Antibody, Unconjugated (bsm-52469R) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Green, bs-0295G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil. Merged staining of anti-CD8A (bsm-34251M; 1:200; pink) anti-CD4 (bsm-52469R; 1:200; light blue) anti-CD3E (bsm-60002R; 1:200; green) anti-CD68 (bsm-60634R; 1:200; white) anti-CD11c (bsm-61135R; 1:200; orange) and anti-CD20 (bsm-63327R; 1:200; yellow) DAPI (dark blue) was used as a nuclear counter stain.


Paraformaldehyde-fixed, paraffin embedded Human lymph nodes. Merged staining of anti-CD8A (bsm-34251M; 1:200; pink) anti-CD4 (bsm-52469R; 1:200; yellow) and anti-CD3E (bsm-60002R; 1:200; light blue) DAPI (dark blue) was used as a nuclear counter stain.


Paraformaldehyde-fixed, paraffin embedded Human Tonsil. Merged staining of anti-CD68 (bsm-60634R; 1:200; red) anti- CD20 (bsm-63327R; 1:200; white) anti-CD3E (bsm-60002R; 1:200; light blue) anti-CD4 (bsm-52469R; 1:200; rose red) anti-Ki67 (bsm-52455R; 1:200; bean green) anti-Vimentin (bsm-33170M; 1:200; yellow) and anti-Pan Cytokeratin (bsm-34137M; 1:150; green) DAPI (dark blue) was used as a nuclear counter stain.