bsm-52479R
[Primary Antibody]
Neuropilin-1 (49H9) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Neuropilin-1
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 8829
Swiss Prot: O14786
Source: Human Neuropilin 1 between 900 to C-terminus
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- E Wen. et al. Tuftsin ameliorates splenic inflammatory injury by promoting neuropilin-1 in severe acute pancreatitis. BIOCHEM PHARMACOL. Biochem Pharmacol. 2022 May;199:11503Read more>>
VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R)) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Mouse heart ; Lane 2: Mouse liver; Lane 3:Mouse kidney; Lane 4 : Human liver; Probed with Neuropilin-1 (49H9)) Monoclonal Antibody (bsm-52479R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neuropilin-1(49H9) ) Monoclonal Antibody, Unconjugated (bsm-52479R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Neuropilin-1 (49H9) Monoclonal Antibody (bsm-52479R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neuropilin-1(49H9) ) Monoclonal Antibody, Unconjugated (bsm-52479R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Lane 1: Mouse Heart Lysates; Lane 2: Mouse Spinal cord Lysates; Lane 3: Rat Kidney Lysates. Probed with Neuropilin-1 monoclonal Antibody, unconjugated (bsm-52479R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.