bsm-52485R-HRP [Conjugated Primary Antibody]
Sodium Potassium ATPase Recombinant Antibody, HRP Conjugated
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Host: Rabbit

Target Protein: Sodium Potassium ATPase

Clonality: Recombinant

Isotype: IgG

Entrez Gene: 476

Swiss Prot: P05023

Source: Human Sodium Potassium ATPase between 1 to 100 amino acids

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Conjugation: HRP

Excitation/ Emission: N/A

Size: 100µL

Concentration: Lot dependent

Applications: WB(1:300-5000)
IHC-P(1:20-100)
IHC-F(1:100-500)

Predicted Molecular Weight: 113


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Sodium Potassium ATPase Monoclonal Antibody (bsm-52485R-HRP) at 1:200 overnight at 4°C, and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Sodium Potassium ATPase monoclonal antibody, HRP conjugated (bsm-52485R-HRP) at 1:5000 dilution and 4°C overnight incubation.