VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Lovo cells; Lane 2: PC-12 cells; Probed with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:100 in LoVo cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse prostate tissue incubated with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.