bsm-52487R [Primary Antibody]
MSI2 (49B4) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: MSI2

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 124540

Swiss Prot: Q96DH6

Source: Human MSI2 between 250 amino acids to the C-terminus

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of HeLa cells with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: Lovo cells; Lane 2: PC-12 cells; Probed with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:100 in LoVo cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Mouse prostate tissue incubated with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with MSI2 (49B4) Monoclonal Antibody (bsm-52487R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.