bsm-52499R [Primary Antibody]
SGK1 (6D1) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SGK1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6446

Swiss Prot: O00141

Source: Human SGK1 aa 1-100

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]

Size: 100ul

Concentration: 1ug/ul

Predicted Molecular Weight: 49


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of 293 cells with SGK1 (6D1) Monoclonal Antibody (bsm-52499R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Human kidney probed with SGK1 (6D1) Monoclonal Antibody (bsm-52499R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C


Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with SGK1 (6D1) Monoclonal Antibody (bsm-52499R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with SGK1 (6D1) Monoclonal Antibody (bsm-52499R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with SGK1 (6D1) Monoclonal Antibody (bsm-52499R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with SGK1 (6D1) Monoclonal Antibody (bsm-52499R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.