DATASHEET
Host:
Rabbit
Target Protein:
Wilms Tumor Protein
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
7490
Swiss Prot:
P19544
Source:
Human Wilms Tumor Protein between 50 to 250 amino acids
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
VALIDATION IMAGES
Flow cytometric analysis of K562 cells with Wilms Tumor Protein (9G6) Monoclonal Antibody (bsm-52515R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
Human kidney lysate probed with Wilms Tumor Protein (9G6) Monoclonal Antibody (bsm-52515R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Wilms Tumor Protein (9G6) Monoclonal Antibody (bsm-52515R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.