VALIDATION IMAGES
Lane 1: K562 Cells; Lane 2: Human skeletal muscle; Probed with Asparagine synthetase (46A1) Monoclonal Antibody (bsm-52520R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Asparagine synthetase (46A1) Monoclonal Antibody (bsm-52520R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Asparagine synthetase (46A1) Monoclonal Antibody (bsm-52520R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Asparagine synthetase (46A1) Monoclonal Antibody (bsm-52520R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with Asparagine synthetase (46A1) Monoclonal Antibody (bsm-52520R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.