bsm-52523R
[Primary Antibody]
Apg7 (13A2) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Apg7
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 10533
Swiss Prot: O95352
Source: Human Apg7 between 1-200 amino acids
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with Apg7 (13A2) Monoclonal Antibody (bsm-52523R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Flow cytometric analysis of HeLa cells with Apg7 (13A2) Monoclonal Antibody (bsm-52523R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Jurkat cell lysate probed with Apg7 (13A2) Monoclonal Antibody (bsm-52523R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Apg7 (13A2) Monoclonal Antibody (bsm-52523R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Apg7 (13A2) Monoclonal Antibody (bsm-52523R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Apg7 (13A2) Monoclonal Antibody (bsm-52523R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Apg7 (13A2) Monoclonal Antibody (bsm-52523R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.