bsm-52529R [Primary Antibody]
Sonic Hedgehog Protein (C-Product) (4C10) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Sonic Hedgehog Protein (C-Product)

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 6469

Swiss Prot: Q15465

Source: Human Sonic Hedgehog between 250-350 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity).

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of HeLa cells with Sonic Hedgehog Protein/SHH(C-Product) (4C10) Monoclonal Antibody (bsm-52529R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: Hela Cells; Lane 2: HepG2 Cells; Probed with Sonic Hedgehog Protein (C-Product) (4C10) Monoclonal Antibody (bsm-52529R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with Sonic Hedgehog Protein/SHH(C-Product) (4C10) Monoclonal Antibody (bsm-52529R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Sonic Hedgehog Protein/SHH(C-Product) (4C10) Monoclonal Antibody (bsm-52529R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with Sonic Hedgehog Protein/SHH(C-Product) (4C10) Monoclonal Antibody (bsm-52529R) at 1:100 in 293 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Sonic Hedgehog Protein/SHH(C-Product) (4C10) Monoclonal Antibody (bsm-52529R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Sonic Hedgehog Protein/SHH(C-Product) (4C10) Monoclonal Antibody (bsm-52529R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Sonic Hedgehog Protein/SHH(C-Product) (4C10) Monoclonal Antibody (bsm-52529R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.