DATASHEET
Host:
Rabbit
Target Protein:
DKK1
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
22943
Swiss Prot:
O94907
Source:
Recombinant Human DKK1 between 250-350 amino acids
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
VALIDATION IMAGES
Flow cytometric analysis of HeLa cells with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
MCF-7 cell lysate probed with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.