bsm-52532R [Primary Antibody]
DKK1 (1B10) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DKK1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 22943

Swiss Prot: O94907

Source: Recombinant Human DKK1 between 250-350 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of HeLa cells with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


MCF-7 cell lysate probed with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with DKK1 (1B10) Monoclonal Antibody (bsm-52532R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.