bsm-52545R [Primary Antibody]
AKAP14 (11A7) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AKAP14

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 158798

Swiss Prot: Q86UN6

Source: Recombinant protein of C terminal human AKAP14

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Binds to type II regulatory subunits of protein kinase A and anchors/targets them.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Jurkat Cells; Lane 2: A549 Cells; Probed with AKAP14 (11A7) Monoclonal Antibody (bsm-52545R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with AKAP14 (11A7) Monoclonal Antibody (bsm-52545R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with AKAP14 (11A7) Monoclonal Antibody (bsm-52545R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.