DATASHEET
Host:
Rabbit
Target Protein:
GAP43
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
2596
Swiss Prot:
P17677
Source:
Recombinant protein within residues on the C-terminus of human GAP43
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.
VALIDATION IMAGES
Flow cytometric analysis of SH-sy5y cells with GAP43 (8H2) Monoclonal Antibody (bsm-52565R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Mouse Brain Probed with GAP43 (8H2) Monoclonal Antibody (bsm-52565R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with GAP43 (8H2) Monoclonal Antibody (bsm-52565R) at 1:300 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.