bsm-52568R [Primary Antibody]
Hes1 (3F8) Monoclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Hes1

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 3280

Swiss Prot: Q14469

Source: Recombinant human Hes1 between 200 to 300 amino acids

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

Size: 100ul

Concentration: 1ug/ul

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Flow cytometric analysis of sh-sy5y cells with Hes1 (3F8) Monoclonal Antibody (bsm-52568R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).


Lane 1: MCF-7 Cells; Lane 2: SH-SY-5Y Cells; Probed with Hes1 (3F8) Monoclonal Antibody (bsm-52568R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.