bsm-52571R
[Primary Antibody]
IKB beta (9D10) Monoclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: IKB beta
Clonality: Monoclonal
Isotype: IgG
Entrez Gene: 4793
Swiss Prot: Q15653
Source: Full length protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
Size: 100ul
Concentration: 1ug/ul
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: THP-1 Cells; Probed with IKB beta (9D10) Monoclonal Antibody (bsm-52571R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with IKB beta (9D10) Monoclonal Antibody (bsm-52571R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with IKB beta (9D10) Monoclonal Antibody (bsm-52571R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with IKB beta (9D10) Monoclonal Antibody (bsm-52571R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.