DATASHEET
Host:
Rabbit
Target Protein:
Myosin Light Chain 2
Clonality:
Monoclonal
Isotype:
IgG
Entrez Gene:
17906
Swiss Prot:
P51667
Source:
Human Myosin Light Chain 2 between 150-250 amino acids
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Contractile protein that plays a role in heart development and function (PubMed:10409661). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity) (PubMed:22426213, PubMed:16908724, PubMed:10409661). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (PubMed:9422794).
VALIDATION IMAGES
Lane 1: Mouse liver; Lane 2: Mouse brain; Probed with Myosin Light Chain 2 (10B7) Monoclonal Antibody (bsm-52575R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed and paraffin-embedded Mouse skeletal muscle tissue incubated with Myosin Light Chain 2 (10B7) Monoclonal Antibody (bsm-52575R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with Myosin Light Chain 2 (10B7) Monoclonal Antibody (bsm-52575R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.