bsm-52591R [Primary Antibody]
MMP11 Recombinant Antibody
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Host: Rabbit

Target Protein: MMP11

Clonality: Monoclonal

Isotype: IgG

Entrez Gene: 4320

Swiss Prot: P24347

Source: Full length protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

May play an important role in the progression of epithelial malignancies.

Size: 100µL

Concentration: Lot Dependent

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
IHC

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse spleen lysate probed with MMP11 (6C1) Monoclonal Antibody (bsm-52591R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


IF(ICC) staining with MMP11 (6C1) Monoclonal Antibody (bsm-52591R) at 1:800 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with MMP11 (6C1) Monoclonal Antibody (bsm-52591R) at 1:800 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


IF(ICC) staining with MMP11 (6C1) Monoclonal Antibody (bsm-52591R) at 1:800 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.


Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with MMP11 (6C1) Monoclonal Antibody (bsm-52591R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with MMP11 (6C1) Monoclonal Antibody (bsm-52591R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.


Paraformaldehyde-fixed and paraffin-embedded Mouse spleen tissue incubated with MMP11 (6C1) Monoclonal Antibody (bsm-52591R) at 1:300, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.