VALIDATION IMAGES
Lane 1: Hela Cells; Lane 2: 293 Cells; Probed with Glutaminase (4A11) Monoclonal Antibody (bsm-52608R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with Glutaminase (4A11) Monoclonal Antibody (bsm-52608R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
IF(ICC) staining with Glutaminase (4A11) Monoclonal Antibody (bsm-52608R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Glutaminase (4A11) Monoclonal Antibody (bsm-52608R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Glutaminase (4A11) Monoclonal Antibody (bsm-52608R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.