VALIDATION IMAGES
Lane 1: A431 Cells; Lane 2: NIH/3T3 Cells; Lane 3: 293T Cells; Probed with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100 in 293 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100 in L6 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat kidney tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4°C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.